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Due to the fact Kc is an element which is wholly depending on a particular column and solvent stream charge, a quantitative measure on the affinity of the compound for a specific set of mobile and stationary phases that does not depend on the column geometry is useful.They recognized 4 protein candidates which were preferentially expressed in LCNEC

Autosampler – draws samples from vials and injects them to the solvent circulation furnished by the pump.From the separation, the molecules working from the column may also be considered as staying in a ongoing equilibrium among the mobile stage as well as stationary section. This equilibrium can be ruled by an equilibrium constant K, defined as

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